Article: article from journal or magazin.
Drosophila melanogaster as a model host to dissect the immunopathogenesis of zygomycosis.
Proceedings of the National Academy of Sciences of the United States of America
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Zygomycosis is an emerging frequently fatal opportunistic mycosis whose immunopathogenesis is poorly understood. We developed a zygomycosis model by injecting Drosophila melanogaster flies with a standardized amount of fungal spores from clinical Zygomycetes isolates to study virulence and host defense mechanisms. We found that, as opposed to most other fungi, which are nonpathogenic in D. melanogaster (e.g., Aspergillus fumigatus), Zygomycetes rapidly infect and kill wild-type flies. Toll-deficient flies exhibited increased susceptibility to Zygomycetes, whereas constitutive overexpression of the antifungal peptide Drosomycin in transgenic flies partially restored resistance to zygomycosis. D. melanogaster Schneider 2 phagocytic cells displayed decreased phagocytosis and caused less hyphal damage to Zygomycetes compared with that to A. fumigatus. Furthermore, phagocytosis-defective eater mutant flies displayed increased susceptibility to Zygomycetes infection. Classic enhancers of Zygomycetes virulence in humans, such as corticosteroids, increased iron supply, and iron availability through treatment with deferoxamine dramatically increased Zygomycetes pathogenicity in our model. In contrast, iron starvation induced by treatment with the iron chelator deferasirox significantly protected flies infected with Zygomycetes. Whole-genome expression profiling in wild-type flies after infection with Zygomycetes vs. A. fumigatus identified genes selectively down-regulated by Zygomycetes, which act in pathogen recognition, immune defense, stress response, detoxification, steroid metabolism, or tissue repair or have unknown functions. Our results provide insights into the factors that mediate host-pathogen interactions in zygomycosis and establish D. melanogaster as a promising model to study this important mycosis.
Animals, Antimicrobial Cationic Peptides/genetics, Antimicrobial Cationic Peptides/metabolism, Aspergillus fumigatus/drug effects, Aspergillus fumigatus/pathogenicity, Dexamethasone/pharmacology, Disease Models, Animal, Disease Susceptibility, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster/drug effects, Drosophila melanogaster/genetics, Fungi/drug effects, Fungi/isolation & purification, Gene Expression Regulation/drug effects, Genes, Insect, Humans, Hyphae/drug effects, Hyphae/physiology, Phagocytes/drug effects, Phagocytes/immunology, Phagocytosis/drug effects, RNA, Messenger/genetics, RNA, Messenger/metabolism, Survival Analysis, Virulence/drug effects, Zygomycosis/immunology, Zygomycosis/pathology
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