Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
Details
Serval ID
serval:BIB_6AC4C4F556F3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
1986
Volume
83
Number
10
Pages
3243-3247
Language
english
Abstract
A genomic 9.3-kilobase DNA fragment of the sea urchin Psammechinus miliaris, containing a cluster of five U7-RNA genes (or pseudogenes), has been isolated and analyzed by partial DNA sequencing. The U7-RNA coding sequences differ from one another by one or two nucleotides, one of the five gene sequences being identical to those of the cDNA U73 clone prepared earlier [Strub, K., Galli, G., Busslinger, M. & Birnstiel, M. L. (1984) EMBO J. 3, 2801-2807]. The spacer sequences separating the genes have, on the whole, a low degree of homology; hence, the five genes must have arisen by an ancient duplication event. The sequences preceding the coding portion contain three highly conserved sequence motifs but no "TATA box." The 3' flanking sequences include a highly conserved AAAGNNAGA sequence that is held in common with other U-RNA genes from both sea urchins and vertebrates. Our findings confirm our classification of the U7 RNA as a genuine, if sparsely represented, member of the U-RNA family.
Keywords
Animals, Enhancer Elements, Genetic, Genes, Genetic Linkage, Histones/genetics, Nucleic Acid Precursors/metabolism, Polymorphism, Genetic, RNA Processing, Post-Transcriptional, RNA, Messenger/metabolism, RNA, Small Nuclear/genetics, Sea Urchins/genetics
Pubmed
Web of science
Create date
22/04/2013 8:30
Last modification date
20/08/2019 14:25
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