Article: article from journal or magazin.
LACK-specific CD4(+) T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection.
Infection and Immunity
The profile of cytokines induced by soluble leishmania antigen (SLA) and the Leishmania homologue of the mammalian receptor for activated C kinase (LACK), a candidate vaccine against leishmaniasis, and the cellular source of the cytokines produced in response to these antigens were analyzed in patients infected with Leishmania guyanensis. Gamma interferon (IFN-gamma) and interleukin-10 (IL-10) were produced in response to LACK. Although LACK-specific CD4(+) cells producing IFN-gamma were isolated only during the early phase of infection (less than 30 days following the onset of infection), cells producing IL-10 in response to LACK were detected in all patients. CD4(+) T cells producing IFN-gamma and IL-13 were produced in response to SLA in all patients. SLA- and LACK-specific T cells are effector memory cells, as they are CD45RA(-) CCR7(-) CD4(+) T cells. CD4(+) T cells producing IFN-gamma are CD62L(-), and CD4(+) T cells producing IL-10 are CD62L(+), indicating that these cells have different tissue-homing capacities. These findings show that SLA and LACK induce both type 1 (IFN-gamma) and type 2 (IL-10 or IL-13) cell responses.
Amino Acid Sequence, Animals, Antigens, CD45, Antigens, Protozoan/immunology, Biological Markers, CD4-Positive T-Lymphocytes/immunology, Cells, Cultured, Humans, Interferon-gamma/biosynthesis, Interleukin-10/secretion, Interleukin-13/biosynthesis, L-Selectin, Leishmania guyanensis/immunology, Leishmaniasis, Mucocutaneous/blood, Leishmaniasis, Mucocutaneous/immunology, Leukocytes, Mononuclear/cytology, Leukocytes, Mononuclear/immunology, Molecular Sequence Data, Peptides/immunology, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Protozoan Proteins/immunology, Receptors, CCR7, Receptors, Chemokine, Time Factors
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