Separation of blood microsamples by exploiting sedimentation at the microscale.

Détails

Ressource 1Télécharger: pmid30237536.pdf (2263.55 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_6A1C20674E95
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Separation of blood microsamples by exploiting sedimentation at the microscale.
Périodique
Scientific reports
Auteur(s)
Forchelet D., Béguin S., Sajic T., Bararpour N., Pataky Z., Frias M., Grabherr S., Augsburger M., Liu Y., Charnley M., Déglon J., Aebersold R., Thomas A., Renaud P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
20/09/2018
Peer-reviewed
Oui
Volume
8
Numéro
1
Pages
14101
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Microsample analysis is highly beneficial in blood-based testing where cutting-edge bioanalytical technologies enable the analysis of volumes down to a few tens of microliters. Despite the availability of analytical methods, the difficulty in obtaining high-quality and standardized microsamples at the point of collection remains a major limitation of the process. Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. Based on this phenomenon, we developed a portable capillary-driven microfluidic device that separates blood microsamples collected from finger-pricks and delivers 2 µL of metered serum for bench-top analysis. Flow cytometric analysis demonstrated the high purity of generated microsamples. Proteomic and metabolomic analyses of the microsamples of 283 proteins and 1351 metabolite features was consistent with samples generated via a conventional centrifugation method. These results were confirmed by a clinical study scrutinising 8 blood markers in obese patients.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/09/2018 9:24
Dernière modification de la notice
08/05/2019 19:55
Données d'usage