In vivo loading increases mechanical properties of scaffold by affecting bone formation and bone resorption rates.

Détails

Ressource 1Demande d'une copie Sous embargo indéterminé.
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_69FC0345634C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo loading increases mechanical properties of scaffold by affecting bone formation and bone resorption rates.
Périodique
Bone
Auteur(s)
Roshan-Ghias A., Lambers F.M., Gholam-Rezaee M., Müller R., Pioletti D.P.
ISSN
1873-2763 (Electronic)
ISSN-L
1873-2763
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
49
Numéro
6
Pages
1357-64
Langue
anglais
Résumé
A successful bone tissue engineering strategy entails producing bone-scaffold constructs with adequate mechanical properties. Apart from the mechanical properties of the scaffold itself, the forming bone inside the scaffold also adds to the strength of the construct. In this study, we investigated the role of in vivo cyclic loading on mechanical properties of a bone scaffold. We implanted PLA/β-TCP scaffolds in the distal femur of six rats, applied external cyclic loading on the right leg, and kept the left leg as a control. We monitored bone formation at 7 time points over 35 weeks using time-lapsed micro-computed tomography (CT) imaging. The images were then used to construct micro-finite element models of bone-scaffold constructs, with which we estimated the stiffness for each sample at all time points. We found that loading increased the stiffness by 60% at 35 weeks. The increase of stiffness was correlated to an increase in bone volume fraction of 18% in the loaded scaffold compared to control scaffold. These changes in volume fraction and related stiffness in the bone scaffold are regulated by two independent processes, bone formation and bone resorption. Using time-lapsed micro-CT imaging and a newly-developed longitudinal image registration technique, we observed that mechanical stimulation increases the bone formation rate during 4-10 weeks, and decreases the bone resorption rate during 9-18 weeks post-operatively. For the first time, we report that in vivo cyclic loading increases mechanical properties of the scaffold by increasing the bone formation rate and decreasing the bone resorption rate.
Pubmed
Web of science
Création de la notice
09/02/2012 11:39
Dernière modification de la notice
03/03/2018 18:00
Données d'usage