Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex.

Détails

ID Serval
serval:BIB_69E2D7CB7009
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex.
Périodique
Journal of Biological Chemistry
Auteur(s)
Chong S.S., Hu P., Hernandez N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2001
Volume
276
Numéro
23
Pages
20727-20734
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human U6 small nuclear (sn) RNA core promoter consists of a proximal sequence element, which recruits the multisubunit factor SNAP(c), and a TATA box, which recruits the TATA box-binding protein, TBP. In addition to SNAP(c) and TBP, transcription from the human U6 promoter requires two well defined factors. The first is hB", a human homologue of the B" subunit of yeast TFIIIB generally required for transcription of RNA polymerase III genes, and the second is hBRFU, one of two human homologues of the yeast TFIIIB subunit BRF specifically required for transcription of U6-type RNA polymerase III promoters. Here, we have partially purified and characterized a RNA polymerase III complex that can direct transcription from the human U6 promoter when combined with recombinant SNAP(c), recombinant TBP, recombinant hB", and recombinant hBRFU. These results open the way to reconstitution of U6 transcription from entirely defined components.
Mots-clé
Amino Acid Sequence, DNA-Binding Proteins/metabolism, Humans, Molecular Sequence Data, Open Reading Frames, Precipitin Tests, Promoter Regions, Genetic, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, RNA, Small Nuclear/metabolism, Recombinant Proteins/metabolism, Sequence Homology, Amino Acid, TATA Box, TATA-Box Binding Protein, Transcription Factors/metabolism
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 15:24
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