Monoclonal antibodies to carcinoembryonic antigen: ionic strength as a factor in the selection of antibodies for immunoscintigraphy.

Details

Serval ID
serval:BIB_69B56867BC09
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Monoclonal antibodies to carcinoembryonic antigen: ionic strength as a factor in the selection of antibodies for immunoscintigraphy.
Journal
Cancer Research
Author(s)
Haskell C.M., Buchegger F., Schreyer M., Carrel S., Mach J.P.
ISSN
0008-5472 (Print)
ISSN-L
0008-5472
Publication state
Published
Issued date
1983
Volume
43
Number
8
Pages
3857-3864
Language
english
Abstract
As part of an ongoing effort to improve the technique of immunoscintigraphy for the detection of human carcinomas with radiolabeled monoclonal antibodies (MABs) to carcinoembryonic antigen (CEA), we have developed a series of MABs to CEA and have studied the effects of low- and physiological molarity buffers on their CEA binding and affinity, as well as their cross-reactivity with granulocyte glycoprotein(s). These in vitro results in different buffer systems were then correlated with the use of these MABs to CEA in the detection of human colon carcinoma grafts in nude mice. Our results show that the binding of CEA by some MABs is influenced by ionic strength and that this may be an important factor in their successful use for the immunolocalization of carcinomas in vivo.
Keywords
Animals, Antibodies, Monoclonal/immunology, Carcinoembryonic Antigen/immunology, Colonic Neoplasms/analysis, Cross Reactions, Immunologic Techniques, Mice, Mice, Nude, Osmolar Concentration, Radionuclide Imaging/methods
Pubmed
Web of science
Create date
25/04/2013 13:54
Last modification date
20/08/2019 14:24
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