Article: article from journal or magazin.
Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin
The canonical Wnt signaling pathway plays key roles in stem-cell maintenance, progenitor cell expansion, and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with lymphoid enhancer factor/T cell factor transcription factors. Here we show that hematopoiesis, including thymopoiesis, is normal in the combined absence of beta- and gamma-catenin. Double-deficient hematopoietic stem cells maintain long-term repopulation capacity and multilineage differentiation potential. Unexpectedly, 2 independent ex vivo reporter gene assays show that Wnt signal transmission is maintained in double-deficient hematopoietic stem cells, thymocytes, or peripheral T cells. In contrast, Wnt signaling is strongly reduced in thymocytes lacking TCF-1 or in nonhematopoietic cells devoid of beta-catenin. These data provide the first evidence that hematopoietic cells can transduce canonical Wnt signals in the combined absence of beta- and gamma-catenin
Animals , beta Catenin , Bone Marrow Cells , Cell Lineage , cytology , gamma Catenin , genetics , Hematopoiesis , Hematopoietic Stem Cells , Hybridomas , metabolism , Mice , Mice,Inbred C57BL , Mice,Transgenic , physiology , Proteins , Role , Signal Transduction , Spleen , Stem Cells , Switzerland , T-Lymphocytes , Thymus Gland , Transcription Factors , transmission , Universities , Wnt Proteins
Web of science
Last modification date