Method validation of nanoparticle tracking analysis to measure pulmonary nanoparticle content: the size distribution in exhaled breath condensate depends on occupational exposure

Details

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_69938B8398C1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Method validation of nanoparticle tracking analysis to measure pulmonary nanoparticle content: the size distribution in exhaled breath condensate depends on occupational exposure
Journal
Journal of Breath Research
Author(s)
Sauvain Jean-Jacques, Suarez Guillaume, Edmé J.L., Bezerra O.M., Silveira K.G., Amaral L.S., Carneiro A.P., Chérot-Kornobis N., Sobaszek A., Hulo S.
ISSN
1752-7163 (Electronic)
ISSN-L
1752-7155
Publication state
Published
Issued date
24/01/2017
Peer-reviewed
Oui
Volume
11
Number
1
Pages
016010
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
A particle exposure assessment based on the dose deposited in the lungs would be the gold standard for the evaluation of any resulting health effects. Measuring particles in exhaled breath condensate (EBC)-a matrix containing water and airway lining fluid-could help to evaluate particle retention in the lungs. This study aimed to (1) validate a nanoparticle tracking analysis (NTA) method for determining the particle number concentration and their hydrodynamic size distribution in EBC, and (2) apply this method to EBC collected from workers exposed to soapstone (n = 55) or quartz dust (n = 12) and controls (n = 11). A standard latex bead solution was used to determine the linear range, limit of detection (LOD), repeatability (coefficient of variation, CV), and bias in spiked EBC. An LM10 NanoSight instrument with NTA version 3.1 software was used for measurement. RTubes(®) were used for field collection of EBC. The repeatability obtained for a D50 size distribution in EBC showed less than 8% variability, with a bias <7%. The particle concentration was linear in the range ≤2.5 × 10(8) particles ml(-1) with a LOD of 4 × 10(6) particles ml(-1). A recovery of 117 ± 20% at 6.2 × 10(7) particles ml(-1) was obtained with a CV <10% and a bias <20%. EBC from workers exposed to quartz, who experienced the largest exposure to silica particles, consistently exhibited a statistically significant (p < 0.01) higher concentration of particles in their EBC, with a size distribution shift towards larger values than the other groups. Results showed that the NTA technique performed well for characterizing the size distribution and concentrations of particles in EBC. The technique needs to be corroborated with a larger population of workers.

Keywords
Nanoparticles/analysis, Breath Tests, Occupational Exposure
Pubmed
Web of science
Open Access
Yes
Create date
30/08/2017 16:36
Last modification date
20/08/2019 14:24
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