Article: article from journal or magazin.
T helper epitopes enhance the cytotoxic response of mice immunized with MHC class I-restricted malaria peptides
Journal of Immunological Methods
Journal Article --- Old month value: Oct 19
We have previously derived MHC class I (H-2Kd) restricted cytotoxic T lymphocytes (CTL) from BALB/c mice immunized with irradiated sporozoites from Plasmodium (P.) berghei and P. yoelii. The CTL recognize synthetic peptides corresponding to a region of the circumsporozoite (CS) protein that is homologous in the two species. In the present study, we have attempted to induce CS-specific CTL by immunization with those peptides in incomplete Freund's adjuvant. Only a low level CTL response was detected in BALB/c mice immunized with synthetic peptides corresponding to the Pb or Py CTL epitopes. In contrast, CS-specific CTL responses could be readily detected in mice injected with mixtures of peptides that combined the P. berghei or P. yoelii CTL epitopes with previously defined T helper epitopes. Several different T helper epitopes were shown to enhance the response when injected as separate peptides in a mixture, or when covalently linked to a CTL epitope. These results may have general implications for the elicitation of CTL responses to defined CTL epitopes and for the design of peptide-based synthetic vaccines.
Amino Acid Sequence Animals Antigens, Protozoan/*immunology Epitopes/*immunology Histocompatibility Antigens Class I/*immunology Immunization Malaria/*immunology Mice Mice, Inbred BALB C Molecular Sequence Data Peptides/chemical synthesis/immunology Plasmodium berghei/immunology Plasmodium yoelii/immunology Protozoan Proteins/immunology T-Lymphocytes, Cytotoxic/*immunology T-Lymphocytes, Helper-Inducer/*immunology
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