Article: article from journal or magazin.
Two-dimensional impedance imaging of cell migration and epithelial stratification
Lab on a Chip
Journal Article --- Old month value: Sep
We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 microm x 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min(-1) following a "scratch" wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.
Cell Culture Techniques Cell Movement/*physiology Cells, Cultured *Electric Impedance Equipment Design Humans Image Processing, Computer-Assisted Infant, Newborn Keratinocytes/*physiology Male Microelectrodes Miniaturization Skin/cytology Time Factors Wound Healing/*physiology
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