Article: article from journal or magazin.
In vivo localization of a bispecific antibody which targets human T lymphocytes to lyse human colon cancer cells.
International Journal of Cancer
A bispecific MAb was derived from the fusion of a hybridoma producing MAb CD3 with a hybridoma producing MAb L-DI (which is directed against a 41-kDa glycoprotein expressed in most gastro-intestinal and pancreatic carcinomas). Bispecific antibody molecules were isolated from parental antibody molecules by the use of hydroxylapatite-HPLC and shown to target human cytolytic T lymphocytes, irrespective of their original specificity, to specifically lyse human colon carcinoma cells. Localization studies in vivo using nude mice bearing human colon carcinoma xenografts showed significant accumulation of the HPLC-purified 125I-labelled bispecific antibodies into the tumor compared to 131I-labelled control CD3 antibody.
Animals, Antibodies, Neoplasm/analysis, Antibodies, Neoplasm/biosynthesis, Chromatography, High Pressure Liquid/methods, Colonic Neoplasms/immunology, Flow Cytometry, Fluorescent Antibody Technique, Humans, Hybridomas/immunology, Mice, Mice, Nude, Neoplasm Transplantation, T-Lymphocytes, Cytotoxic/immunology
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