Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_68DBA46C664C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
Périodique
Plos One
Auteur(s)
Boss D., Hoffmann A., Rappaz B., Depeursinge C., Magistretti P.J., Van de Ville D., Marquet P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
7
Numéro
8
Pages
e40667
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of these remarkable biomechanical membrane properties altered in some pathological states including blood diseases. In particular the discussion over the thermal or metabolic origin of the CMF has led in the past to a large number of investigations and modeling. However, the origin of the CMF is still debated. In this article, we present an analysis of the CMF of RBCs by combining digital holographic microscopy (DHM) with an orthogonal subspace decomposition of the imaging data. These subspace components can be reliably identified and quantified as the eigenmode basis of CMF that minimizes the deformation energy of the RBC structure. By fitting the observed fluctuation modes with a theoretical dynamic model, we find that the CMF are mainly governed by the bending elasticity of the membrane and that shear and tension elasticities have only a marginal influence on the membrane fluctations of the discocyte RBC. Further, our experiments show that the role of ATP as a driving force of CMF is questionable. ATP, however, seems to be required to maintain the unique biomechanical properties of the RBC membrane that lead to thermally excited CMF.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/09/2012 13:33
Dernière modification de la notice
20/08/2019 14:23
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