Functional analysis of bacteriophage f1 intergenic region.

Details

Serval ID
serval:BIB_68A86A2176D5
Type
Article: article from journal or magazin.
Collection
Publications
Title
Functional analysis of bacteriophage f1 intergenic region.
Journal
Virology
Author(s)
Dotto G.P., Enea V., Zinder N.D.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Publication state
Published
Issued date
1981
Volume
114
Number
2
Pages
463-473
Language
english
Abstract
The bacteriophage fl genome contains an intergenic region (IG) 508 nucleotides long that does not code for any known protein. By using chimeric plasmids harboring different portions of this region as well as neighboring parts of the fl genome, we have identified three functional domains: (1) the proximal part of gene II, responsible for the prevention of fl and f2 adsorption and penetration (IP2 phenotype); (2) a part in the middle of the IG responsible for the fl "functional origin of replication;" and (3) a sequence at the gene IV/IG junction important for particle morphogenesis. We have shown that these regions cab be inserted into separate sites of a plasmid and function independently of one another.
Keywords
Adsorption, Base Sequence, Coliphages/genetics, Coliphages/growth & development, DNA Replication, Genes, Viral, Morphogenesis, Transduction, Genetic, Virus Replication
Pubmed
Web of science
Create date
24/01/2008 15:59
Last modification date
20/08/2019 15:23
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