Different sensitivities to pH of ATP-induced currents at four cloned P2X receptors.

Détails

ID Serval
serval:BIB_680A4140DEEA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Different sensitivities to pH of ATP-induced currents at four cloned P2X receptors.
Périodique
Journal of Neurophysiology
Auteur(s)
Stoop R., Surprenant A., North R.A.
ISSN
0022-3077 (Print)
ISSN-L
0022-3077
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
78
Numéro
4
Pages
1837-1840
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The effect of changing extracellular pH was studied on the currents induced by ATP or alphabeta-methylene-ATP in HEK293 cells transfected with different P2X receptor subunits. In cells expressing P2X1, P2X3, or P2X4 receptors, the effect of ATP was decreased by acidification. In cells expressing P2X2 receptors, acidification increased the ATP-induced current; this effect was also seen in cells expressing heteromeric P2X2 and P2X3 receptors. At P2X2 receptors, acidification caused a leftward shift in the ATP concentration-response curve, without change in maximum; the pKa for this effect was 7.3. At P2X4 receptors, acidification caused a rightward shift in the ATP concentration-response curve, without change in the maximum; the pKa for this effect was 6.8. The pH dependence of the action of ATP should be taken into account in studies of synaptic transmission, and it may provide a further tool to assign molecular identity to P2X receptors expressed by brain neurons.
Mots-clé
Adenosine Triphosphate/pharmacology, Animals, Cloning, Molecular, Dose-Response Relationship, Drug, Hydrogen-Ion Concentration, Patch-Clamp Techniques, Receptors, Purinergic P2/drug effects, Sensitivity and Specificity, Synaptic Transmission/drug effects, Synaptic Transmission/physiology
Pubmed
Création de la notice
29/10/2013 10:46
Dernière modification de la notice
20/08/2019 14:23
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