The cells and peripheral representation of sodium taste in mice.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_67FF1B8C2279
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The cells and peripheral representation of sodium taste in mice.
Périodique
Nature
Auteur(s)
Chandrashekar J., Kuhn C., Oka Y., Yarmolinsky D.A., Hummler E., Ryba N.J., Zuker C.S.
ISSN
1476-4687
ISSN-L
0028-0836
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
464
Numéro
7286
Pages
297-301
Langue
anglais
Résumé
Salt taste in mammals can trigger two divergent behavioural responses. In general, concentrated saline solutions elicit robust behavioural aversion, whereas low concentrations of NaCl are typically attractive, particularly after sodium depletion. Notably, the attractive salt pathway is selectively responsive to sodium and inhibited by amiloride, whereas the aversive one functions as a non-selective detector for a wide range of salts. Because amiloride is a potent inhibitor of the epithelial sodium channel (ENaC), ENaC has been proposed to function as a component of the salt-taste-receptor system. Previously, we showed that four of the five basic taste qualities-sweet, sour, bitter and umami-are mediated by separate taste-receptor cells (TRCs) each tuned to a single taste modality, and wired to elicit stereotypical behavioural responses. Here we show that sodium sensing is also mediated by a dedicated population of TRCs. These taste cells express the epithelial sodium channel ENaC, and mediate behavioural attraction to NaCl. We genetically engineered mice lacking ENaCalpha in TRCs, and produced animals exhibiting a complete loss of salt attraction and sodium taste responses. Together, these studies substantiate independent cellular substrates for all five basic taste qualities, and validate the essential role of ENaC for sodium taste in mice.
Mots-clé
Animals, Behavior/physiology, Epithelial Sodium Channel/genetics, Epithelial Sodium Channel/metabolism, Mice, Mice, Transgenic, Sodium/physiology, Taste/genetics, Taste Buds/cytology, Taste Buds/metabolism
Pubmed
Web of science
Création de la notice
18/11/2010 16:48
Dernière modification de la notice
20/08/2019 14:23
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