The fusion protein SS18-SSX1 employs core Wnt pathway transcription factors to induce a partial Wnt signature in synovial sarcoma.

Détails

Ressource 1Télécharger: pmid26905812.pdf (2024.07 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_66B69F702FDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The fusion protein SS18-SSX1 employs core Wnt pathway transcription factors to induce a partial Wnt signature in synovial sarcoma.
Périodique
Scientific reports
Auteur(s)
Cironi L., Petricevic T., Fernandes Vieira V., Provero P., Fusco C., Cornaz S., Fregni G., Letovanec I., Aguet M., Stamenkovic I.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
24/02/2016
Peer-reviewed
Oui
Volume
6
Pages
22113
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication types: Journal Article ; Research Support, Non-U.S. Gov't

Résumé
Expression of the SS18/SYT-SSX fusion protein is believed to underlie the pathogenesis of synovial sarcoma (SS). Recent evidence suggests that deregulation of the Wnt pathway may play an important role in SS but the mechanisms whereby SS18-SSX might affect Wnt signaling remain to be elucidated. Here, we show that SS18/SSX tightly regulates the elevated expression of the key Wnt target AXIN2 in primary SS. SS18-SSX is shown to interact with TCF/LEF, TLE and HDAC but not β-catenin in vivo and to induce Wnt target gene expression by forming a complex containing promoter-bound TCF/LEF and HDAC but lacking β-catenin. Our observations provide a tumor-specific mechanistic basis for Wnt target gene induction in SS that can occur in the absence of Wnt ligand stimulation.

Mots-clé
Animals, Axin Protein/genetics, Axin Protein/metabolism, Blotting, Western, Cell Line, Cell Line, Tumor, Gene Expression Profiling/methods, Histone Deacetylases/genetics, Histone Deacetylases/metabolism, Humans, Mice, Microscopy, Confocal, Oncogene Proteins, Fusion/genetics, Oncogene Proteins, Fusion/metabolism, RNA Interference, Repressor Proteins/genetics, Repressor Proteins/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, Synovial/genetics, Sarcoma, Synovial/metabolism, Sarcoma, Synovial/pathology, TCF Transcription Factors/genetics, TCF Transcription Factors/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Wnt Signaling Pathway/genetics, beta Catenin/genetics, beta Catenin/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/02/2016 19:49
Dernière modification de la notice
20/08/2019 15:22
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