Article: article from journal or magazin.
Unresponsiveness to lymphoid-mediated signals at the neonatal follicular dendritic cell precursor level contributes to delayed germinal center induction and limitations of neonatal antibody responses to T-dependent antigens
Journal of Immunology
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Mar 15
The factors limiting neonatal and infant IgG Ab responses to T-dependent Ags are only partly known. In this study, we assess how these B cell responses are influenced by the postnatal development of the spleen and lymph node microarchitecture. When BALB/c mice were immunized with alum-adsorbed tetanus toxoid at various stages of their immune development, a major functional maturation step for induction of serum IgG, Ab-secreting cells, and germinal center (GC) responses was identified between the second and the third week of life. This correlated with the development of the follicular dendritic cell (FDC) network, as mature FDC clusters only appeared at 2 wk of age. Adoptive transfer of neonatal splenocytes into adult SCID mice rapidly induced B cell follicles and FDC precursor differentiation into mature FDC, indicating effective recruitment and signaling capacity of neonatal B cells. In contrast, adoptive transfer of adult splenocytes into neonatal SCID mice induced primary B cell follicles without any differentiation of mature FDC and failed to correct limitations of tetanus toxoid-induced GC. Thus, unresponsiveness to lymphoid-mediated signals at the level of neonatal FDC precursors delays FDC maturation and GC induction, thus limiting primary Ab-secreting cell responses to T-dependent Ags in early postnatal life.
Aging/immunology Animals Animals, Newborn/growth & development/*immunology Antibodies, Bacterial/biosynthesis Antibody-Producing Cells/cytology/*secretion B-Lymphocyte Subsets/cytology/immunology Cell Differentiation/immunology Cell Movement/immunology Chemokines, CXC/physiology Dendritic Cells, Follicular/cytology/*immunology Female Germinal Center/cytology/*immunology Haptens/immunology Immune Tolerance/*immunology Lymphocyte Subsets/cytology/*immunology Mice Mice, Inbred BALB C Mice, SCID Ovalbumin/immunology Receptors, Chemokine Receptors, Cytokine/physiology Signal Transduction/*immunology Stem Cells/cytology/*immunology Tetanus Toxoid/immunology Trinitrobenzenes/immunology
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