Malaria vaccine: immunization of mice with a synthetic T cell helper epitope alone leads to protective immunity
Details
Serval ID
serval:BIB_6690A9336337
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Malaria vaccine: immunization of mice with a synthetic T cell helper epitope alone leads to protective immunity
Journal
European Journal of Immunology
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
02/1993
Volume
23
Number
2
Pages
582-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
The immunogenicity of the non-repetitive sequences of the Plasmodium berghei circumsporozoite (CS) protein was studied using synthetic peptides. Two CS sequences (residues 20-39 and 57-70) exhibiting T cell helper activity were identified. Immunization of BALB/c mice with a branched peptide containing either the 20-39 or the 57-70 sequence and two repeats (B epitope) in a linear sequence induced high titers of anti-repeat and anti-sporozoite antibodies. Mice immunized with the T-B construct (high antibody titers) or with the 57-70 epitope alone (no serum anti-repeat or anti-peptide antibodies) were protected to a similar degree after challenge with infective sporozoites. No protection was obtained in mice immunized with the 20-39 epitope. These results indicate that BALB/c mice can be protected either by effector T cells or by high levels of anti-repeat antibodies. Thus, in the same strain, a double mechanism of protection can be obtained by a synthetic peptide vaccine.
Keywords
Amino Acid Sequence
Animals
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Epitopes/*immunology
Immunity
Malaria/immunology/*prevention & control
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Molecular Sequence Data
Plasmodium berghei/*immunology
Protozoan Proteins/chemical synthesis/immunology
*Protozoan Vaccines
T-Lymphocytes/*immunology
T-Lymphocytes, Helper-Inducer/*immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 14:22