Malaria vaccine: immunization of mice with a synthetic T cell helper epitope alone leads to protective immunity

Details

Serval ID
serval:BIB_6690A9336337
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Malaria vaccine: immunization of mice with a synthetic T cell helper epitope alone leads to protective immunity
Journal
European Journal of Immunology
Author(s)
Migliorini  P., Betschart  B., Corradin  G.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
02/1993
Volume
23
Number
2
Pages
582-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
The immunogenicity of the non-repetitive sequences of the Plasmodium berghei circumsporozoite (CS) protein was studied using synthetic peptides. Two CS sequences (residues 20-39 and 57-70) exhibiting T cell helper activity were identified. Immunization of BALB/c mice with a branched peptide containing either the 20-39 or the 57-70 sequence and two repeats (B epitope) in a linear sequence induced high titers of anti-repeat and anti-sporozoite antibodies. Mice immunized with the T-B construct (high antibody titers) or with the 57-70 epitope alone (no serum anti-repeat or anti-peptide antibodies) were protected to a similar degree after challenge with infective sporozoites. No protection was obtained in mice immunized with the 20-39 epitope. These results indicate that BALB/c mice can be protected either by effector T cells or by high levels of anti-repeat antibodies. Thus, in the same strain, a double mechanism of protection can be obtained by a synthetic peptide vaccine.
Keywords
Amino Acid Sequence Animals Cells, Cultured Enzyme-Linked Immunosorbent Assay Epitopes/*immunology Immunity Malaria/immunology/*prevention & control Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Molecular Sequence Data Plasmodium berghei/*immunology Protozoan Proteins/chemical synthesis/immunology *Protozoan Vaccines T-Lymphocytes/*immunology T-Lymphocytes, Helper-Inducer/*immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 14:22
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