Conferences are an integral part of academia, providing researchers with the opportunity to collaborate and disseminate their research. Traditionally these conferences have been in-person, but COVID-19 restrictions and growing recognition of systematic barriers have led many academic societies to reimagine the conference format. Social media platforms are often used to promote and broadcast in-person academic conferences, so with the development of online conferences they have naturally become hosting platforms. For example, during the peak of COVID-19 restrictions, when in-person meetings were not possible, Twitter conferences, like the first Global Animal Behaviour Conference (#AnimBehav2021), provided a much needed platform to collaborate and share science. Recently, conferences have returned to an in-person format. In addition, a change in ownership of Twitter led to widespread restructuring of the Twitter workforce and misinformation management policies that resulted in a massive exodus of academics from the platform. During these changes, we organized and held the second Global Animal Behaviour Twitter Conference (#AnimBehav2023) in January 2023. In this paper, we report (1) how engagement in the Twitter conference changed as a result of return to in-person conferences and changes in Twitter ownership, (2) how the community views online conferences in general and Twitter conferences in particular and (3) reflect on how online conferences can be organized moving forward. Given the widespread benefits of and need for online conferences, we urge academic societies and researchers to continue systematically exploring these alternative conference formats to ensure inclusivity and accessibility in academic spaces.