PAR-1 is required for the maintenance of oocyte fate in Drosophila.

Détails

ID Serval
serval:BIB_6612569EABF0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
PAR-1 is required for the maintenance of oocyte fate in Drosophila.
Périodique
Development
Auteur(s)
Huynh J.R., Shulman J.M., Benton R., St Johnston D.
ISSN
0950-1991[print], 0950-1991[linking]
Statut éditorial
Publié
Date de publication
2001
Volume
128
Numéro
7
Pages
1201-1209
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The PAR-1 kinase is required for the posterior localisation of the germline determinants in C. elegans and Drosophila, and localises to the posterior of the zygote and the oocyte in each case. We show that Drosophila PAR-1 is also required much earlier in oogenesis for the selection of one cell in a germline cyst to become the oocyte. Although the initial steps in oocyte determination are delayed, three markers for oocyte identity, the synaptonemal complex, the centrosomes and Orb protein, still become restricted to one cell in mutant clones. However, the centrosomes and Orb protein fail to translocate from the anterior to the posterior cortex of the presumptive oocyte in region 3 of the germarium, and the cell exits meiosis and becomes a nurse cell. Furthermore, markers for the minus ends of the microtubules also fail to move from the anterior to the posterior of the oocyte in mutant clones. Thus, PAR-1 is required for the maintenance of oocyte identity, and plays a role in microtubule-dependent localisation within the oocyte at two stages of oogenesis. Finally, we show that PAR-1 localises on the fusome, and provides a link between the asymmetry of the fusome and the selection of the oocyte.
Mots-clé
Animals, Biological Markers, Caenorhabditis elegans Proteins, Cell Differentiation, Cell Fusion, Drosophila/physiology, Drosophila Proteins, Kinesin, Meiosis, Microtubule Proteins/metabolism, Microtubules/metabolism, Mutagenesis, Oocytes/cytology, Oogenesis/physiology, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Recombinant Fusion Proteins/metabolism
Pubmed
Web of science
Création de la notice
18/03/2008 10:36
Dernière modification de la notice
20/08/2019 14:21
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