Monoclonal antibodies directed against the amino-terminal domain of human TBP cross-react with TBP from other species.

Détails

ID Serval
serval:BIB_65C3A4AF5C2C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Monoclonal antibodies directed against the amino-terminal domain of human TBP cross-react with TBP from other species.
Périodique
Hybridoma
Auteur(s)
Ruppert S.M., McCulloch V., Meyer M., Bautista C., Falkowski M., Stunnenberg H.G., Hernandez N.
ISSN
0272-457X
Statut éditorial
Publié
Date de publication
02/1996
Peer-reviewed
Oui
Volume
15
Numéro
1
Pages
55-68
Langue
anglais
Résumé
The TATA box-binding protein (TBP) is a key transcription factor required for transcription by all three eukaryotic RNA polymerases. It consists of a conserved carboxy-terminal DNA binding domain and a highly divergent amino terminal domain. TBP and different sets of TBP-associated factors (TAFs) constitute at least four multisubunit complexes referred to as SL1, TFIID, TFIIIB, and SNAPC. SL1, TFIID, and TFIIIB are required for transcription by RNA polymerases I, II, and III, respectively, while the SNAP complex is involved in transcription of the small nuclear RNA (snRNA) genes by RNA polymerases II and III. TBP also associates with a number of basal transcription factors such as TFIIA and TFIIB, and with several regulatory factors such as VP16, E1A, and p53. Here we describe the characterization of a panel of monoclonal antibodies (MAbs) directed against the amino-terminal domain of human TBP. These MAbs recognize different TBP epitopes, some of which have been precisely defined. Different MAbs recognize different TBP-containing complexes and several of them crossreact with TBP from other species. These antibodies can be used to purify TBP-containing complexes in a functional form and should be useful to identify new protein-protein interactions involving TBP.
Mots-clé
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Conserved Sequence, Cricetinae, Cross Reactions, DNA, DNA-Binding Proteins, Drosophila, Electrophoresis, Polyacrylamide Gel, Epitope Mapping, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptide Fragments, Pol1 Transcription Initiation Complex Proteins, Rats, Sea Urchins, Species Specificity, TATA-Box Binding Protein, Transcription Factors
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 15:21
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