Article: article from journal or magazin.
The TPTE gene family: cellular expression, subcellular localization and alternative splicing.
The human TPTE (Transmembrane Phosphatase with TEnsin homology) gene family encodes a PTEN-related tyrosine phosphatase with four potential transmembrane domains. Chromosomal mapping revealed multiple copies of the TPTE gene on chromosomes 13, 15, 21, 22 and Y. Human chromosomes 13 and 21 copies encode two functional proteins, TPIP (TPTE and PTEN homologous Inositol lipid Phosphatase) and TPTE, respectively, whereas only one copy of the gene exists in the mouse genome. In the present study, we show that TPTE and TPIP proteins are expressed in secondary spermatocytes and/or prespermatids. In addition, we report the existence of several novel alternatively spliced isoforms of these two proteins with variable number of transmembrane domains. The latter has no influence on the subcellular localization of these different peptides as shown by co-immunofluorescence experiments. Finally, we identify another expressed TPTE copy, mapping to human chromosome 22, whose transcription appears to be under the control of the LTR of human endogenous retrovirus RTVL-H3.
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, COS Cells, Cell Line, Cercopithecus aethiops, Chromosomes, Human, Pair 22/genetics, DNA, Complementary/chemistry, DNA, Complementary/genetics, Female, Gene Expression Regulation, Enzymologic, Green Fluorescent Proteins, Hela Cells, Humans, Immunohistochemistry, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Male, Membrane Proteins/genetics, Membrane Proteins/metabolism, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, PTEN Phosphohydrolase, Phosphoric Monoester Hydrolases/genetics, Phosphoric Monoester Hydrolases/metabolism, Protein Isoforms/genetics, Protein Isoforms/metabolism, Protein Tyrosine Phosphatases/genetics, Protein Tyrosine Phosphatases/metabolism, Pseudogenes/genetics, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Sequence Analysis, DNA, Spermatids/enzymology, Spermatocytes/enzymology, Testicular Neoplasms/enzymology, Testicular Neoplasms/genetics, Testis/enzymology
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