Holliday junction branch migration and resolution assays

Détails

ID Serval
serval:BIB_65934DB80F25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Holliday junction branch migration and resolution assays
Périodique
Methods in Molecular Biology
Auteur(s)
Constantinou  A., West  S. C.
ISSN
1064-3745 (Print)
Statut éditorial
Publié
Date de publication
2004
Volume
262
Pages
239-53
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
Holliday junctions are central intermediates in the process of genetic recombination; they form as a consequence of a reciprocal exchange of strands between paired DNA molecules. Enzymes that specifically recognize and process these junctions are necessary for the formation of recombinant products. In the methods described here, we detail the in vitro construction of two types of Holliday junction: (1) a small synthetic junction formed by the annealing of partially complementary oligonucleotides; and (2) a true recombination intermediate structure formed by RecA protein-mediated strand exchange. The use of these substrates in assays designed to detect Holliday junction branch migration and resolution activities is described.
Mots-clé
Base Sequence Centrifugation, Density Gradient/methods DNA, Circular/genetics/isolation & purification DNA, Cruciform/chemistry/*physiology DNA, Single-Stranded/genetics/isolation & purification Genetic Techniques Models, Genetic Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Plasmids/genetics *Recombination, Genetic
Pubmed
Création de la notice
24/01/2008 15:50
Dernière modification de la notice
03/03/2018 17:52
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