Critical involvement of Tcf-1 in expansion of thymocytes.

Détails

ID Serval
serval:BIB_6592
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Critical involvement of Tcf-1 in expansion of thymocytes.
Périodique
Journal of Immunology
Auteur(s)
Schilham M.W., Wilson A., Moerer P., Benaissa-Trouw B.J., Cumano A., Clevers H.C.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1998
Volume
161
Numéro
8
Pages
3984-3991
Langue
anglais
Résumé
T cell maturation in Tcf-1(-/-) mice deteriorates progressively and halts completely around 6 mo of age. During fetal development thymocyte subpopulations seem normal, although total cell numbers are lower. By 4 to 6 wk of age, obvious blockades in the differentiation of CD4- 8- thymocytes are observed at two distinct stages (CD44+ 25+ and CD44- 25-), both of which are normally characterized by extensive proliferation. This lack of thymocyte expansion and/or differentiation was also observed when Tcf-1(-/-) progenitor cells from the aorta-gonad-mesonephros region (embryonic day 11.5), fetal liver (embryonic day 12.5/14.5), and fetal bone marrow (embryonic day 18.5) were allowed to differentiate in normal thymic lobes (fetal thymic organ cultures) or were injected intrathymically into normal recipients. Despite these apparent defects in thymocyte differentiation and expansion, adult Tcf-1(-/-) mice are immunocompetent, as they generate virus neutralizing Abs at normal titers. Furthermore, their peripheral T cells have an activated phenotype (increased CD44 and decreased CD62L expression) and proliferate normally in response to Ag or mitogen, suggesting that these cells may have arisen from the early wave of development during embryogenesis and are either long lived or have subsequently been maintained by peripheral expansion. As Tcf-1 is a critical component in the Wnt/beta-catenin signaling pathway, these data suggest that Wnt-like factors play a role in the expansion of double-negative thymocytes.
Mots-clé
Animals, Antigens, CD/immunology, Cell Differentiation/genetics, Cell Differentiation/immunology, DNA-Binding Proteins/genetics, DNA-Binding Proteins/immunology, Gene Expression Regulation/immunology, Lymphoid Enhancer-Binding Factor 1, Mice, Mice, Transgenic, T Cell Transcription Factor 1, T-Lymphocytes/cytology, T-Lymphocytes/immunology, Transcription Factors/genetics, Transcription Factors/immunology
Pubmed
Web of science
Création de la notice
19/11/2007 12:43
Dernière modification de la notice
20/08/2019 14:21
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