Article: article from journal or magazin.
TCR-MHC class II interaction is required for peripheral expansion of CD4 cells in a T cell-deficient host.
Publication types: Journal Article Publication Status: ppublish
It is well established that T cell-deficient nude and SCID mice can be reconstituted by i.v. injection of small numbers of purified peripheral CD4+ T cells; however, the requirements for expansion of the transferred T cells in such systems are not clear. We show here that blood and lymphoid organs of MHC class II-deficient mice (which selectively lack mature CD4+ T cells) cannot be reconstituted by transfer of purified splenic CD4+ T cells, whereas TCRalpha-deficient mice (which lack both CD4+ and CD8+ mature T cells) are readily reconstituted. The failure of CD4+ T cell reconstitution in MHC class II-deficient mice was not due to the presence of CD8+ T cells, since similar results were obtained in TCRalpha-MHC class II double-deficient mice. Consistent with most previous studies CD4+ T cells in reconstituted TCRalpha-deficient mice had a diverse TCR Vbeta repertoire and were predominantly of an activated/memory (CD44high) phenotype. Collectively our data demonstrate that the expansion of peripheral CD4+ T cells in a T cell-deficient host is dependent upon interactions of the TCR with MHC class II.
Animals, CD4-Positive T-Lymphocytes/physiology, Histocompatibility Antigens Class II/physiology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Nude, Mice, SCID, Receptors, Antigen, T-Cell, alpha-beta/physiology
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