Alterations of Brain Energy Metabolism in Type 2 Diabetic Goto-Kakizaki Rats Measured In Vivo by <sup>13</sup>C Magnetic Resonance Spectroscopy.

Détails

ID Serval
serval:BIB_65093A7C02C0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Alterations of Brain Energy Metabolism in Type 2 Diabetic Goto-Kakizaki Rats Measured In Vivo by <sup>13</sup>C Magnetic Resonance Spectroscopy.
Périodique
Neurotoxicity research
Auteur(s)
Girault F.M., Sonnay S., Gruetter R., Duarte JMN
ISSN
1476-3524 (Electronic)
ISSN-L
1029-8428
Statut éditorial
Publié
Date de publication
08/2019
Peer-reviewed
Oui
Volume
36
Numéro
2
Pages
268-278
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Type 2 diabetes (T2D) is associated with deterioration of brain structure and function. Here, we tested the hypothesis that T2D induces a reorganization of the brain metabolic networks that support brain function. For that, alterations of neuronal and glial energy metabolism were investigated in a T2D model, the Goto-Kakizaki (GK) rat. <sup>13</sup> C magnetic resonance spectroscopy in vivo at 14.1 T was used to detect <sup>13</sup> C labeling incorporation into carbons of glutamate, glutamine, and aspartate in the brain of GK (n = 7) and Wistar (n = 13) rats during intravenous [1,6- <sup>13</sup> C]glucose administration. Labeling of brain glucose and amino acids over time was analyzed with a two-compartment mathematical model of brain energy metabolism to determine the rates of metabolic pathways in neurons and glia. Compared to controls, GK rats displayed lower rates of brain glutamine synthesis (- 32%, P < 0.001) and glutamate-glutamine cycle (- 40%, P < 0.001), and mitochondrial tricarboxylic acid (TCA) cycle rate in neurons (- 7%, P = 0.036). In contrast, the TCA cycle rate of astrocytes was larger in GK rats than controls (+ 21%, P = 0.042). We conclude that T2D alters brain energy metabolism and impairs the glutamate-glutamine cycle between neurons and astrocytes, in line with diabetes-induced neurodegeneration and astrogliosis underlying brain dysfunction.
Mots-clé
Brain energy metabolism, Glucose, Insulin resistance, Magnetic resonance spectroscopy, Neuron-glia interactions
Pubmed
Web of science
Création de la notice
09/10/2017 14:24
Dernière modification de la notice
21/08/2019 6:15
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