Shedding light on the expansion and diversification of the Cdc48 protein family during the rise of the eukaryotic cell.

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Version: Final published version
Serval ID
serval:BIB_6507E37B9686
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Shedding light on the expansion and diversification of the Cdc48 protein family during the rise of the eukaryotic cell.
Journal
BMC evolutionary biology
Author(s)
Kienle N., Kloepper T.H., Fasshauer D.
ISSN
1471-2148 (Electronic)
ISSN-L
1471-2148
Publication state
Published
Issued date
18/10/2016
Peer-reviewed
Oui
Volume
16
Number
1
Pages
215
Language
english
Notes
Publication types: ARTICLE
Publication Status: epublish
Publication types: Journal Article

Abstract
A defining feature of eukaryotic cells is the presence of various distinct membrane-bound compartments with different metabolic roles. Material exchange between most compartments occurs via a sophisticated vesicle trafficking system. This intricate cellular architecture of eukaryotes appears to have emerged suddenly, about 2 billion years ago, from much less complex ancestors. How the eukaryotic cell acquired its internal complexity is poorly understood, partly because no prokaryotic precursors have been found for many key factors involved in compartmentalization. One exception is the Cdc48 protein family, which consists of several distinct classical ATPases associated with various cellular activities (AAA+) proteins with two consecutive AAA domains.
Here, we have classified the Cdc48 family through iterative use of hidden Markov models and tree building. We found only one type, Cdc48, in prokaryotes, although a set of eight diverged members that function at distinct subcellular compartments were retrieved from eukaryotes and were probably present in the last eukaryotic common ancestor (LECA). Pronounced changes in sequence and domain structure during the radiation into the LECA set are delineated. Moreover, our analysis brings to light lineage-specific losses and duplications that often reflect important biological changes. Remarkably, we also found evidence for internal duplications within the LECA set that probably occurred during the rise of the eukaryotic cell.
Our analysis corroborates the idea that the diversification of the Cdc48 family is closely intertwined with the development of the compartments of the eukaryotic cell.

Pubmed
Open Access
Yes
Create date
01/11/2016 19:40
Last modification date
20/08/2019 14:21
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