Article: article from journal or magazin.
Intrathecal immune responses to EBV in early MS.
European Journal of Immunology
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
EBV has been consistently associated with MS, but its signature in the CNS has rarely been examined. In this study, we assessed EBV-specific humoral and cellular immune responses in the cerebrospinal fluid (CSF) of patients with early MS, other inflammatory neurological diseases (OIND) and non-inflammatory neurological diseases (NIND). The neurotropic herpesvirus CMV served as a control. Virus-specific humoral immune responses were assessed in 123 consecutive patients and the intrathecal recruitment of virus-specific antibodies was expressed as antibody indexes. Cellular immune responses tested in the blood of 55/123 patients were positive in 46/55. The CD8(+) CTL responses of these 46 patients were assessed in the blood and CSF using a CFSE-based CTL assay. We found that viral capsid antigen and EBV-encoded nuclear antigen-1, but not CMV IgG antibody indexes, were increased in early MS as compared with OIND and NIND patients. There was also intrathecal enrichment in EBV-, but not CMV-specific, CD8(+) CTL in early MS patients. By contrast, OIND and NIND patients did not recruit EBV- nor CMV-specific CD8(+) CTL in the CSF. Our data, showing a high EBV-, but not CMV-specific intrathecal immune response, strengthen the association between EBV and MS, in particular at the onset of the disease.
Adult, Antibodies, Viral/blood, Antibodies, Viral/cerebrospinal fluid, Antigens, Viral/immunology, Capsid Proteins/immunology, Cell Separation, Epstein-Barr Virus Infections/complications, Epstein-Barr Virus Infections/immunology, Epstein-Barr Virus Nuclear Antigens/immunology, Flow Cytometry, Herpesvirus 4, Human/immunology, Humans, Immunoglobulin G/blood, Immunoglobulin G/cerebrospinal fluid, Multiple Sclerosis/blood, Multiple Sclerosis/cerebrospinal fluid, T-Lymphocytes, Cytotoxic/immunology
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