Diversity of staphylococcal cassette chromosome mec elements in predominant methicillin-resistant Staphylococcus aureus clones in a small geographic area.

Détails

ID Serval
serval:BIB_64AD5F769E34
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Diversity of staphylococcal cassette chromosome mec elements in predominant methicillin-resistant Staphylococcus aureus clones in a small geographic area.
Périodique
Antimicrobial Agents and Chemotherapy
Auteur(s)
Basset P., Senn L., Vogel V., Zanetti G., Blanc D.S.
ISSN
1098-6596[electronic], 0066-4804[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
54
Numéro
11
Pages
4589-4595
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Recent population genetic studies suggest that staphylococcal cassette chromosome mec (SCCmec) was acquired much more frequently than previously thought. In the present study, we aimed to investigate the diversity of SCCmec elements in a local methicillin-resistant Staphylococcus aureus (MRSA) population. Each MRSA isolate (one per patient) recovered in the Vaud canton of Switzerland from January 2005 to December 2008 was analyzed by the double-locus sequence typing (DLST) method and SCCmec typing. DLST analysis indicated that 1,884/2,036 isolates (92.5%) belong to four predominant clones. As expected from the local spread of a clone, most isolates within clones harbored an identical SCCmec type. However, three to seven SCCmec types have been recovered in every predominant DLST clone, suggesting that some of these elements might have been acquired locally. This pattern could also be explained by distinct importations of related isolates into the study region. The addition of a third highly variable locus to further increase the discriminatory power of typing as well as epidemiological data suggested that most ambiguous situations were explained by the second hypothesis. In conclusion, our study showed that even if the acquisition of new SCCmec elements at a local level likely occurs, it does not explain all the diversity observed in the study region.
Mots-clé
Chromosomes, Bacterial/genetics, Humans, Methicillin-Resistant Staphylococcus aureus/classification, Methicillin-Resistant Staphylococcus aureus/genetics, Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/12/2010 15:26
Dernière modification de la notice
20/08/2019 14:20
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