Initiation and termination of phage f1 plus-strand synthesis.

Details

Serval ID
serval:BIB_64A612738BDA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Initiation and termination of phage f1 plus-strand synthesis.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Dotto G.P., Horiuchi K., Zinder N.D.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
1982
Volume
79
Number
23
Pages
7122-7126
Language
english
Abstract
The origin of DNA replication of bacteriophage f1 contains a nucleotide sequence that is used both for the initiation of viral (plus) strand synthesis and for its termination. With chimeric plasmids containing two f1 functional origins in the same orientation, synthesis of chimeric plus-strand DNA is initiated, after f1 infection, at either one of the two f1 origins and is terminated at the other. Thus, the chimeric plasmids segregate into two replicons, each of them containing only one f1 origin. This system has been used to test several fragments of the f1 origin varying in size or in nucleotide sequence for their ability to function in either initiation or termination of viral strand synthesis. Our data show that the f1 origin is composed of two overlapping but distinct domains (signals), one for initiation and the other for termination of plus-strand synthesis.
Keywords
Base Sequence, Coliphages/genetics, DNA Replication, DNA, Viral/genetics, Plasmids, Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:59
Last modification date
20/08/2019 14:20
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