Organelle acidification negatively regulates vacuole membrane fusion in vivo.

Détails

ID Serval
serval:BIB_649CBFF23C97
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Organelle acidification negatively regulates vacuole membrane fusion in vivo.
Périodique
Scientific reports
Auteur(s)
Desfougères Y., Vavassori S., Rompf M., Gerasimaite R., Mayer A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
01/07/2016
Peer-reviewed
Oui
Volume
6
Pages
29045
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
The V-ATPase is a proton pump consisting of a membrane-integral V0 sector and a peripheral V1 sector, which carries the ATPase activity. In vitro studies of yeast vacuole fusion and evidence from worms, flies, zebrafish and mice suggested that V0 interacts with the SNARE machinery for membrane fusion, that it promotes the induction of hemifusion and that this activity requires physical presence of V0 rather than its proton pump activity. A recent in vivo study in yeast has challenged these interpretations, concluding that fusion required solely lumenal acidification but not the V0 sector itself. Here, we identify the reasons for this discrepancy and reconcile it. We find that acute pharmacological or physiological inhibition of V-ATPase pump activity de-acidifies the vacuole lumen in living yeast cells within minutes. Time-lapse microscopy revealed that de-acidification induces vacuole fusion rather than inhibiting it. Cells expressing mutated V0 subunits that maintain vacuolar acidity were blocked in this fusion. Thus, proton pump activity of the V-ATPase negatively regulates vacuole fusion in vivo. Vacuole fusion in vivo does, however, require physical presence of a fusion-competent V0 sector.
Mots-clé
Acids/metabolism, Biological Transport/genetics, Membrane Fusion/genetics, Membranes/metabolism, SNARE Proteins/genetics, SNARE Proteins/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Vacuolar Proton-Translocating ATPases/genetics, Vacuolar Proton-Translocating ATPases/metabolism, Vacuoles/genetics, Vacuoles/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/07/2016 13:45
Dernière modification de la notice
20/08/2019 14:20
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