The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens.

Détails

ID Serval
serval:BIB_644E639A8682
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Lettre (letter): communication adressée à l'éditeur.
Collection
Publications
Titre
The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens.
Périodique
Plant Signaling and Behavior
Auteur(s)
Saidi Y., Schaefer D.G., Goloubinoff P., Zrÿd J.P., Finka A.
ISSN
1559-2324[electronic], 1559-2316[linking]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
4
Numéro
5
Pages
457-459
Langue
anglais
Notes
Publication types: Comment ; Journal Article
Publication Status: ppublish
Résumé
The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in dark grown old moss cells and complete lack of labelling in newly differentiated cells. Furthermore, we demonstrate that stable moss lines harbouring a resistance cassette driven by the CaMV 35S are unable to grow in darkness in the presence of the antibiotic. In contrast to the CaMV 35S, the heat inducible promoter, hsp17.3B showed uniform expression pattern in all cells and tissues following a mild heat shock.
Mots-clé
F-actin, constitutive/inducible promoter, heat shock, hsp17.3B, sodium salicylate
Pubmed
Open Access
Oui
Création de la notice
23/11/2009 14:18
Dernière modification de la notice
20/08/2019 15:20
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