Processing of human prosomatostatin in AtT-20 cells: S-28 and S-14 are generated in different secretory pathways

Details

Serval ID
serval:BIB_644B3A6B3DBA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Processing of human prosomatostatin in AtT-20 cells: S-28 and S-14 are generated in different secretory pathways
Journal
Biochemical and Biophysical Research Communications
Author(s)
Brakch  N., Cohen  P., Boileau  G.
ISSN
0006-291X (Print)
Publication state
Published
Issued date
11/1994
Volume
205
Number
1
Pages
221-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 30
Abstract
Somatostatin-14 (S-14) and somatostatin-28 (S-28) are generated by differential processing of a single precursor at a dibasic (R-K) or monobasic (R) proteolytic cleavage site, respectively. To study the pathways of processing of prosomatostatin, we have expressed in AtT20 cells cDNA encoding human prosomatostatin and prosomatostatin mutated in one or the other processing site. Analysis of the peptides present in cell extracts or culture media before and after stimulation of the cells with 8-BrcAMP indicated that prosomatostatin can enter three distinct secretory pathways where it is differently processed: 1) prosomatostatin was secreted through the constitutive pathway; 2) the regulated secretory pathway generated S-14 which was released upon stimulation of the cells with 8-BrcAMP; 3) an alternative pathway, insensitive to 8-BrcAMP produced S-28 and S-14. Moreover, our results suggest that the R-K processing site used to produce S-14 is an important structural feature for targeting the precursor to the regulated secretory pathway.
Keywords
8-Bromo Cyclic Adenosine Monophosphate/pharmacology Cell Line DNA, Complementary Humans Protein Precursors/*metabolism/secretion *Protein Processing, Post-Translational Somatostatin/*metabolism/*secretion Somatostatin-28
Pubmed
Web of science
Create date
28/01/2008 10:34
Last modification date
20/08/2019 14:20
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