Virosome-bound antigen enhances DC-dependent specific CD4(+) T cell stimulation, inducing a Th1 and Treg profile in vitro
Details
Serval ID
serval:BIB_639415F908F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Virosome-bound antigen enhances DC-dependent specific CD4(+) T cell stimulation, inducing a Th1 and Treg profile in vitro
Journal
Nanomedicine
ISSN
1549-9642 (Electronic)
ISSN-L
1549-9634
Publication state
Published
Issued date
07/2017
Volume
13
Number
5
Pages
1725-1737
Language
english
Notes
Blom, Rebecca A M
Amacker, Mario
Moser, Christian
van Dijk, R Maarten
Bonetti, Raffaela
Seydoux, Emilie
Hall, Sean R R
von Garnier, Christophe
Blank, Fabian
eng
Nanomedicine. 2017 Jul;13(5):1725-1737. doi: 10.1016/j.nano.2017.02.004. Epub 2017 Feb 15.
Amacker, Mario
Moser, Christian
van Dijk, R Maarten
Bonetti, Raffaela
Seydoux, Emilie
Hall, Sean R R
von Garnier, Christophe
Blank, Fabian
eng
Nanomedicine. 2017 Jul;13(5):1725-1737. doi: 10.1016/j.nano.2017.02.004. Epub 2017 Feb 15.
Abstract
There is considerable interest to develop antigen-carriers for immune-modulatory clinical applications, but insufficient information is available on their effects on antigen-presenting cells. We employed virosomes coupled to ovalbumin (OVA) to study their interaction with murine bone marrow-derived dendritic cells (BMDCs) and modulation of downstream T cell responses. BMDCs were treated in vitro with virosomes or liposomes prior to determining BMDC phenotype, viability, and intracellular trafficking. Antigen-specific CD4(+) T cell activation was measured by co-culture of BMDCs with DO11.10 CD4(+) T cells. Compared to liposomes, virosomes were rapidly taken up. Neither nanocarrier type affected BMDC viability, nor did a moderate degree of activation differ for markers such as CD40, CD80, CD86. Virosome uptake occurred via clathrin-mediated endocytosis and phagocytosis, with co-localization in late endosomes. Only BMDCs treated with OVA-coupled virosomes induced enhanced OVA-specific CD4(+) T cell proliferation. Antigen-coupled virosomes are endowed with an intrinsic ability to modulate DC-dependent adaptive immune responses.
Keywords
Adaptive Immunity, Animals, Antigens, *CD4-Positive T-Lymphocytes, *Dendritic Cells, Mice, Mice, Inbred BALB C, Ovalbumin, T-Lymphocytes, Regulatory, Th1 Cells, *Virosomes, *CD4(+) T cells, *Immune modulation, *Liposomes, *Mouse dendritic cells
Pubmed
Create date
15/04/2021 9:58
Last modification date
01/05/2021 5:33