Induction of the putative protective protein ferritin by infrared radiation: implications in skin repair

Détails

ID Serval
serval:BIB_6388C2A0363D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Induction of the putative protective protein ferritin by infrared radiation: implications in skin repair
Périodique
International Journal of Molecular Medicine
Auteur(s)
Applegate  L. A., Scaletta  C., Panizzon  R., Frenk  E., Hohlfeld  P., Schwarzkopf  S.
ISSN
1107-3756 (Print)
Statut éditorial
Publié
Date de publication
03/2000
Volume
5
Numéro
3
Pages
247-51
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
The modification of ferritin in human skin cells in vitro and in vivo following infrared-A irradiation by immunohistochemical analysis and ELISA were evaluated. In addition, we observed that IR-A is not capable of inducing frank damage to DNA (pyrimidine dimers, p53), induction of oxidative stress proteins (heme oxygenase, nitric oxide, superoxide dismutase, heat shock proteins) or proteases (collagenase, stromelysin, gelatinase) involved in carcinogenesis and photoaging of the skin. in vivo, basal levels of ferritin were heterogeneous for all individuals tested but all showed ferritin to stain precisely in the basal layer of unirradiated epidermis. Following IR-A radiation, the ferritin increase was localized to epidermal tissue and showed an increase from 120 to 220%. Parallel to the in vivo analysis, dermal fibroblasts were cultured from six individuals. Quantitative analysis for ferritin in cultured fibroblasts was assessed by ELISA and increases were seen to be dose-dependent and up to 130% of basal levels of ferritin following infrared-A. Our findings indicate that the putative defense system of ferritin that exists in human skin in vivo can be induced by infrared-A radiation and that these wavelengths may prove to be beneficial for human skin. Importantly, following the same doses of IR-A that induced ferritin levels, there was no alteration seen for nuclear DNA type damage, oxidative stress proteins or proteases involved in the degradation of skin. The increased concentrations of this antioxidant in human skin following acute UV radiation could afford increased protection against subsequent oxidative stress.
Mots-clé
Adult Cells, Cultured Collagenases/biosynthesis DNA Damage Female Ferritins/*biosynthesis Gelatinases/biosynthesis HSP70 Heat-Shock Proteins/biosynthesis Heme Oxygenase (Decyclizing)/biosynthesis Humans Male Matrix Metalloproteinase 3/biosynthesis Middle Aged Nitric Oxide/biosynthesis Oxidative Stress Pyrimidine Dimers/biosynthesis Skin/cytology/metabolism/*radiation effects Tumor Suppressor Protein p53/biosynthesis *Ultraviolet Rays
Pubmed
Web of science
Création de la notice
25/01/2008 16:55
Dernière modification de la notice
20/08/2019 14:20
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