Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Détails

ID Serval
serval:BIB_6356B784BE95
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Submicrometer optical tomography by multiple-wavelength digital holographic microscopy
Périodique
Applied Optics
Auteur(s)
Montfort Frédéric, Colomb Tristan, Charrière Florian, Kühn Jonas, Marquet Pierre, Cuche Etienne, Herminjard Sylvain, Depeursinge Christian
ISSN
0003-6935
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
45
Numéro
32
Pages
8209-8217
Langue
anglais
Notes
SAPHIRID:67333
Résumé
We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic specimen and magnified by a microscope objective. A CCD camera records the holograms consecutively, which are then numerically reconstructed following the convolution formulation to obtain each corresponding complex object wavefront. Their relative phases are adjusted to be equal in a given plane of interest and the resulting complex wavefronts are summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Tomography is thus obtained by the attenuation of the amplitude out of the plane of interest. Numerical variation of the plane of interest enables one to scan the object in depth. For the presented simulations and experiments, 20 wavelengths are used in the 480-700 nm range. The result is a sectioning of the object in slices 725 nm thick.
Pubmed
Web of science
Création de la notice
10/03/2008 16:40
Dernière modification de la notice
20/08/2019 14:19
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