FBI-1, a factor that binds to the HIV-1 inducer of short transcripts (IST), is a POZ domain protein.

Détails

ID Serval
serval:BIB_633AA7F469B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
FBI-1, a factor that binds to the HIV-1 inducer of short transcripts (IST), is a POZ domain protein.
Périodique
Nucleic Acids Research
Auteur(s)
Morrison D.J., Pendergrast P.S., Stavropoulos P., Colmenares S.U., Kobayashi R., Hernandez N.
ISSN
0305-1048[print], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
03/1999
Volume
27
Numéro
5
Pages
1251-1262
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The HIV-1 promoter directs the synthesis of two classes of transcripts, short, non-polyadenylated transcripts and full-length, polyadenylated transcripts. The synthesis of short transcripts is activated by a bipartite DNA element, the inducer of short transcripts or IST, located downstream of the HIV-1 transcriptional start site, while the synthesis of full-length transcripts is activated by the viral activator Tat. Tat binds to the RNA element TAR, which is encoded largely between the two IST half-elements. Upon activation by Tat, the synthesis of short RNAs is repressed. We have previously purified a factor called FBI-1 (for factor that binds to IST) whose binding to wild-type and mutated ISTs correlated well with the abilities of these ISTs to direct the synthesis of short transcripts. Here, we report the cloning of cDNAs encoding FBI-1. FBI-1 contains a POZ domain at its N-terminus and four Krüppel-type zinc fingers at its C-terminus. The C-terminus is sufficient for specific binding, and FBI-1 can form homomers through its POZ domain and, in vivo, through its zinc finger domain as well. In addition, FBI-1 associates with Tat, suggesting that repression of the short transcripts by Tat may be mediated through interactions between the two factors.
Mots-clé
Amino Acid Sequence, DNA, Complementary, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Gene Products, tat/metabolism, HIV-1/genetics, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, RNA, Messenger/genetics, Recombinant Proteins/chemistry, Recombinant Proteins/genetics, Sequence Homology, Amino Acid, Solutions, Zinc Fingers, tat Gene Products, Human Immunodeficiency Virus
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:19
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