Article: article from journal or magazin.
Chlamydiaceae family, Parachlamydia spp., and Waddlia spp. in porcine abortion.
Journal of Veterinary Diagnostic Investigation
At present, despite extensive laboratory investigations, most cases of porcine abortion remain without an etiological diagnosis. Due to a lack of recent data on the abortigenic effect of order Chlamydiales, 286 fetuses and their placentae of 113 abortion cases (1-5 fetuses per abortion case) were investigated by polymerase chain reaction (PCR) methods for family Chlamydiaceae and selected Chlamydia-like organisms such as Parachlamydia acanthamoebae and Waddlia chondrophila. In 0.35% of the cases (1/286 fetuses), the Chlamydiaceae real-time PCR was positive. In the Chlamydiaceae-positive fetus, Chlamydia abortus was detected by a commercial microarray and 16S ribosomal RNA PCR followed by sequencing. The positive fetus had a Porcine circovirus-2 coinfection. By the Parachlamydia real-time PCR, 3.5% (10/286 fetuses of 9 abortion cases) were questionable positive (threshold cycle values: 35.0-45.0). In 2 of these 10 cases, a confirmation by Chlamydiales-specific real-time PCR was possible. All samples tested negative by the Waddlia real-time PCR. It seems unlikely that Chlamydiaceae, Parachlamydia, and Waddlia play an important role as abortigenic agents in Swiss sows.
Aborted Fetus/microbiology, Abortion, Veterinary/microbiology, Animals, Chlamydiales/isolation & purification, DNA, Bacterial/classification, Female, Gram-Negative Bacterial Infections/microbiology, Gram-Negative Bacterial Infections/veterinary, Pregnancy, Pregnancy Complications, Infectious/microbiology, Pregnancy Complications, Infectious/veterinary, Real-Time Polymerase Chain Reaction, Species Specificity, Swine, Swine Diseases/microbiology
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