Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2.

Details

Serval ID
serval:BIB_624C627C9F57
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2.
Journal
EMBO journal
Author(s)
Steiner P., Alberi S., Kulangara K., Yersin A., Sarria J.C., Regulier E., Kasas S., Dietler G., Muller D., Catsicas S., Hirling H.
ISSN
0261-4189
Publication state
Published
Issued date
08/2005
Peer-reviewed
Oui
Volume
24
Number
16
Pages
2873-2884
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Abstract
Trafficking of AMPA-type glutamate receptors (AMPAR) between endosomes and the postsynaptic plasma membrane of neurons plays a central role in the control of synaptic strength associated with learning and memory. The molecular mechanisms of its regulation remain poorly understood, however. Here we show by biochemical and atomic force microscopy analyses that NEEP21, a neuronal endosomal protein necessary for receptor recycling including AMPAR, is associated with the scaffolding protein GRIP1 and the AMPAR subunit GluR2. Moreover, the interaction between NEEP21 and GRIP1 is regulated by neuronal activity. Expression of a NEEP21 fragment containing the GRIP1-binding site decreases surface GluR2 levels and delays recycling of internalized GluR2, which accumulates in early endosomes and lysosomes. Infusion of this fragment into pyramidal neurons of hippocampal slices induces inward rectification of AMPAR-mediated synaptic responses, suggesting decreased GluR2 expression at synapses. These results indicate that NEEP21-GRIP1 binding is crucial for GluR2-AMPAR sorting through endosomes and their recruitment to the plasma membrane, providing a first molecular mechanism to differentially regulate AMPAR subunit cycling in internal compartments.
Keywords
Animals, Carrier Proteins/metabolism, Cell Membrane/metabolism, Chromatography, Gel, Electrophysiology, Endosomes/metabolism, Fluorescent Antibody Technique, Gene Expression Regulation, Hippocampus/metabolism, Immunoprecipitation, Microscopy, Atomic Force, Nerve Tissue Proteins/metabolism, Neurons/metabolism, Protein Transport/physiology, Rats, Receptors, AMPA/metabolism, Synapses/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:24
Last modification date
20/08/2019 14:19
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