Differential gene expression analysis in a rabbit model of osteoarthritis induced by anterior cruciate ligament (ACL) section.

Détails

ID Serval
serval:BIB_6223C5EE052D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differential gene expression analysis in a rabbit model of osteoarthritis induced by anterior cruciate ligament (ACL) section.
Périodique
Biorheology
Auteur(s)
Bluteau G., Gouttenoire J., Conrozier T., Mathieu P., Vignon E., Richard M., Herbage D., Mallein-Gerin F.
ISSN
0006-355X (Print)
ISSN-L
0006-355X
Statut éditorial
Publié
Date de publication
2002
Volume
39
Numéro
1-2
Pages
247-258
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Osteoarthritis (OA) is the most common of all joint diseases to affect mankind and is characterized by the degradation of articular cartilage. The low availability of normal and pathologic human cartilage and the inability to study the early stages of the disease in humans has led to the development of numerous animal models of OA. The aim of our study was to establish gene expression profiles during the progression of a rabbit model of OA induced by anterior cruciate ligament (ACL) section. Semiquantitative RT-PCR was used to follow expression of several relevant molecules (type II and X collagens, aggrecan, osteonectin, betaig-h3, BiP, TIMP-1, MMP-1, -3, -13, aggrecanase-1, -2) during development of OA in articular cartilage. In parallel, we monitored the activities of collagenase, caseinase, phospholipase A2 and glycosyltransferases (xylosyl-, galactosyl-, glucuronyl- and N-acetyl-galactosaminyl-transferase). Novel cDNA clones for rabbit type X collagen, aggrecanase-1 and -2, osteonectin and BiP were constructed to obtain species-specific primers. Ours result show that MMP-13 (collagenase-3) gene expression increased dramatically early after ACL surgery and remained high thereafter. An increase in MMP-1 (collagenase-1) and MMP-3 expression was also noted with an absence of variation for TIMP-1 expression. In addition, the global MMPs activities paralleled the MMP gene expression. These data together characterize at the molecular level the evolution of OA in this rabbit model. Furthermore, we have undertaken a search for identifying differentially expressed genes in normal and OA cartilage in this model, by differential display RT-PCR. We present here preliminary results with the determination of the best technical conditions to obtain reproducible electrophoresis patterns of differential display RT-PCR.
Mots-clé
Aggrecans, Amino Acid Sequence, Animals, Anterior Cruciate Ligament/injuries, Base Sequence, Collagen/genetics, Collagenases/genetics, Disease Models, Animal, Endopeptidases/genetics, Extracellular Matrix Proteins, Gene Expression Profiling, Hindlimb, Lectins, C-Type, Matrix Metalloproteinases/genetics, Metalloendopeptidases/genetics, Molecular Sequence Data, Osteoarthritis/genetics, Osteoarthritis/metabolism, Osteonectin/genetics, Proteoglycans/genetics, Rabbits, Reverse Transcriptase Polymerase Chain Reaction, Tissue Inhibitor of Metalloproteinases/genetics
Pubmed
Création de la notice
01/10/2015 16:21
Dernière modification de la notice
20/08/2019 15:19
Données d'usage