Application de la technique d'hybridation in situ fluorescente (FISH) au diagnostic de la translocation de Philadelphie dans la leucémie myéloïde chronique [Fluorescent in-situ hybridization technique (FISH) in the diagnosis of Philadelphia translocation in chronic myeloid leukemia]

Details

Serval ID
serval:BIB_6211
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Application de la technique d'hybridation in situ fluorescente (FISH) au diagnostic de la translocation de Philadelphie dans la leucémie myéloïde chronique [Fluorescent in-situ hybridization technique (FISH) in the diagnosis of Philadelphia translocation in chronic myeloid leukemia]
Journal
Schweizerische Medizinische Wochenschrift = Journal Suisse de Médecine
Author(s)
Martinet D., Mühlematter D., Jotterand Bellomo M.
ISSN
0036-7672[print], 0036-7672[linking]
Publication state
Published
Issued date
1996
Volume
126
Number
20
Pages
855-863
Language
french
Notes
Publication types: English Abstract ; Journal Article ; Review
Publication Status: ppublish
Abstract
The Philadelphia chromosome (Ph) resulting from translocation t(9;22)(q34;q11) is observed in more than 90% of patients with chronic myeloid leukemia (CML). Its molecular consequence is the genesis of a fusion gene BCR-ABL between the 5' sequences of the BCR gene (chromosome 22) and the 3' end of the ABL gene (chromosome 9). Fluorescence in situ hybridization (FISH) using specific DNA probes provides a useful tool for the detection of t(9;22) and BCR-ABL rearrangement. We report our results using the FISH technique for t(9;22) assessment in the hematopoietic cells of patients with Ph-positive CML. The DNA libraries pBS 9 and pBS 22 containing multiple sequences derived from chromosomes 9 and 22 have been used to identify t(9;22) in metaphase cells. The cos bcr-51 and cos abl-18 probes that hybridize to unique sequences specific to the BCR and ABL genes have the ability to detect the BCR-ABL rearrangement in metaphase cells as well as in interphase nuclei. FISH is a sensitive and specific technique that represents a valuable complement to conventional cytogenetics. The BCR-ABL rearrangement can be detected in metaphase spreads of insufficient quality or from interphase nuclei in the case of terminally differentiated cells or of cells which do not divide in vitro. When the efficiency of hybridization and detection is good, a large number of cells can be analyzed. This is of major significance in assessment of response to treatment and definition of a cytogenetic remission. However, interphase cytogenetics may be difficult due to variations in signal resolution and background level. The FISH technique can also be used to detect the BCR-ABL rearrangement in cases of Ph negative BCR-ABL positive CML.
Keywords
Color, DNA Probes, Fusion Proteins, bcr-abl/isolation &amp, purification, Humans, In Situ Hybridization, Fluorescence/methods, Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics, Philadelphia Chromosome, Translocation, Genetic
Pubmed
Web of science
Create date
19/11/2007 12:43
Last modification date
20/08/2019 14:19
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