Quantitative RT-PCR distribution of serotonin 5-HT6 receptor mRNA in the central nervous system of control or 5,7-dihydroxytryptamine-treated rats

Details

Serval ID
serval:BIB_6208D657D682
Type
Article: article from journal or magazin.
Collection
Publications
Title
Quantitative RT-PCR distribution of serotonin 5-HT6 receptor mRNA in the central nervous system of control or 5,7-dihydroxytryptamine-treated rats
Journal
Synapse
Author(s)
Gerard  C., el Mestikawy  S., Lebrand  C., Adrien  J., Ruat  M., Traiffort  E., Hamon  M., Martres  M. P.
ISSN
0887-4476 (Print)
Publication state
Published
Issued date
07/1996
Volume
23
Number
3
Pages
164-73
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Possible adaptive changes of the recently cloned serotonin 5-HT6 receptor after the selective lesion of serotoninergic neurons by an intracerebral administration of 5,7-dihydroxytryptamine were investigated using competitive RT-PCR (reverse transcription followed by polymerase chain reaction) for the measurement of 5-HT6-mRNA in various areas of the rat central nervous system. In control rats, 5-HT6-mRNA was the most abundant in the nucleus accumbens, followed by the olfactory tubercle and the striatum. High levels of 5-HT6-mRNA were also found in the hypothalamus and the hippocampus, whereas the cerebral cortex, the substantia nigra, and the spinal cord contained moderate levels of the transcript. Low but easily quantifiable levels of 5-HT6-mRNA were measured in the ventral tegmental area, the anterior raphe area, and the cerebellum. In addition, moderate to low levels of this mRNA were also found in dorsal root ganglia and the pituitary gland. Three weeks after the microinfusion of 5,7-dihydroxytryptamine into the anteroventral vicinity of the dorsal raphe nucleus in nomifensine-pretreated rats, the levels of serotonin transporter-mRNA were reduced by 90% in the anterior raphe area, as expected of the extensive lesion of serotoninergic neurons. In contrast, quantitative determinations of the 5-HT6-mRNA in this area as well as in the nucleus accumbens, the striatum, and the hippocampus indicated that its levels were not significantly different in 5,7-dihydroxytryptamine-treated rats and in controls. These data showed that the 5-HT6 receptor: 1) is not an autoreceptor, and 2) exhibits probably no up regulation in postsynaptic target cells after the selective degeneration of serotoninergic projections.
Keywords
5,7-Dihydroxytryptamine/*pharmacology Animals Central Nervous System/drug effects/*metabolism In Situ Hybridization Male Oligonucleotides, Antisense/pharmacology Polymerase Chain Reaction RNA, Messenger/*metabolism Rats Rats, Sprague-Dawley Receptors, Serotonin/*biosynthesis/drug effects Serotonin Agents/*pharmacology Transcription, Genetic/drug effects Up-Regulation/drug effects
Pubmed
Web of science
Create date
24/01/2008 14:27
Last modification date
20/08/2019 14:19
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