A regulated two-step mechanism of TBP binding to DNA: a solvent-exposed surface of TBP inhibits TATA box recognition.

Détails

ID Serval
serval:BIB_61A0A358F2F8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A regulated two-step mechanism of TBP binding to DNA: a solvent-exposed surface of TBP inhibits TATA box recognition.
Périodique
Cell
Auteur(s)
Zhao X., Herr W.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
2002
Volume
108
Numéro
5
Pages
615-627
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S. Publication Status: ppublish
Résumé
The TATA box binding protein TBP plays a universally important role in eukaryotic nuclear transcription. By mutagenesis, we have discovered a solvent-exposed surface of the structured TBP core domain that is important for inhibition of the DNA binding and DNA-bending activities of full-length wild-type TBP. Full-length wild-type TBP initially binds the TATA box to form an unstable complex containing unbent DNA, and then it slowly forms a stable complex containing bent DNA. TFIIB greatly accelerates formation of a bent TFIIB-TBP-TATA box complex, and the inhibitory DNA binding surface of TBP contributes to the cooperativity of binding to TFIIB. Using TBP and TFIIB, we show that TBP can bind the TATA box through a regulated two-step mechanism, involving a transition from unbent complex to bent complex.
Mots-clé
DNA/chemistry, DNA/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Humans, Macromolecular Substances, Models, Genetic, Models, Molecular, Mutation, Nucleic Acid Conformation, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins/metabolism, TATA Box, TATA-Box Binding Protein, Transcription Factor TFIIB, Transcription Factors/chemistry, Transcription Factors/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
08/05/2019 19:26
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