DNA-RNA hybridization
Details
Serval ID
serval:BIB_616F787B9053
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
DNA-RNA hybridization
Journal
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
ISSN
0962-8436 (Print)
Publication state
Published
Issued date
11/1975
Volume
272
Number
915
Pages
147-57
Notes
Journal Article --- Old month value: Nov 6
Abstract
Interest in nucleic acid hybridization stems mainly from its great power as a tool in biological research. It is used in several quite distinct ways. Because of the high degree of specificity that they show, hybridization techniques can be used to measure the amount of one specific sequence within a very heterogeneous mixture of sequences. Measurements of 1/10(6)-10(7) have been recorded. In extension of this, various properties of a specific sequence can often be studied. Secondly, because the kinetics of nucleic acid hybridization are quite well understood, it can be used to characterize both a pure sequence and a very complex mixture of sequences, like the genome of a vertebrate. Thirdly, again because of its specificity, it can be used to measure homologies between different populations of nucleic acids. Lastly, in conjunction with other techniques, it can be used as a basis for the fractionation of nucleic acid populations and the purification of specific sequences. Specific examples of these applications are given, with special reference to the organization of the genome in higher eukaryotes.
Keywords
Animals
Base Sequence
Cell Line
Chromosomes/analysis
DNA/analysis
Drosophila melanogaster
Ducks
Genes
Hemoglobins
Kinetics
*Nucleic Acid Hybridization
Poly A/analysis
RNA Viruses/analysis
RNA, Messenger/analysis
RNA, Viral/analysis
Rabbits
Pubmed
Web of science
Create date
25/01/2008 17:18
Last modification date
20/08/2019 15:18