Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.

Détails

Ressource 1Télécharger: s41467-019-08345-4.pdf (6482.07 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_616D5C56C195
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.
Périodique
Nature communications
Auteur(s)
Chai P.C., Cruchet S., Wigger L., Benton R.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
07/02/2019
Peer-reviewed
Oui
Volume
10
Numéro
1
Pages
643
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Nervous systems exhibit myriad cell types, but understanding how this diversity arises is hampered by the difficulty to visualize and genetically-probe specific lineages, especially at early developmental stages prior to expression of unique molecular markers. Here, we use a genetic immortalization method to analyze the development of sensory neuron lineages in the Drosophila olfactory system, from their origin to terminal differentiation. We apply this approach to define a fate map of nearly all olfactory lineages and refine the model of temporal patterns of lineage divisions. Taking advantage of a selective marker for the lineage that gives rise to Or67d pheromone-sensing neurons and a genome-wide transcription factor RNAi screen, we identify the spatial and temporal requirements for Pointed, an ETS family member, in this developmental pathway. Transcriptomic analysis of wild-type and Pointed-depleted olfactory tissue reveals a universal requirement for this factor as a switch-like determinant of fates in these sensory lineages.
Mots-clé
Animals, Drosophila, Drosophila Proteins/metabolism, Olfactory Bulb/cytology, Olfactory Bulb/metabolism, Sensory Receptor Cells/cytology, Sensory Receptor Cells/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/05/2018 16:57
Dernière modification de la notice
20/08/2019 15:18
Données d'usage