Determination of cutoff values to detect small aneuploid clones by interphase fluorescence in situ hybridization: the Poisson model is a more appropriate approach. Should single-cell trisomy 8 be considered a clonal defect?
Details
Serval ID
serval:BIB_614ED91C79BB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination of cutoff values to detect small aneuploid clones by interphase fluorescence in situ hybridization: the Poisson model is a more appropriate approach. Should single-cell trisomy 8 be considered a clonal defect?
Journal
Cancer Genetics and Cytogenetics
ISSN
0165-4608 (Print)
Publication state
Published
Issued date
12/2003
Volume
147
Number
2
Pages
99-109
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
We applied a dual-color interphase in situ fluorescence hybridization (I-FISH) technique using centromeric probes specific to chromosomes 7 and 8 on 20 control samples in order to define the statistical model best suited to determine cutoff values for detection of small abnormal clones. We found that the Poisson model is a more appropriate approach than a Gaussian model. Then, based on the analysis of 91 samples from 80 patients with myelocytic malignant hemopathies and either clonal or nonclonal -7 or +8 as determined with conventional cytogenetics (CC), we compared the respective power of I-FISH and CC for detection of aneuploidy, with special emphasis on the potential contribution of I-FISH as a complement to CC in the case of small abnormal clones. The I-FISH results were positive in samples with clonal -7 or +8 according to CC analysis. Whereas I-FISH was negative in samples with nonclonal -7 according to CC, thus confirming the reliability of the criteria used to define the clonality of -7; the situation was different with nonclonal +8. I-FISH revealed the clonality of +8 in most samples with single-cell +8. In several cases, however, the unquestionable clonal nature of +8, as evidenced during follow-up, could not be established with either CC or I-FISH according to accepted criteria. Our data suggest that, in case of a single metaphase with +8, the general rule should be amended and the single-cell +8 should be considered and reported as potentially clonal.
Keywords
Aneuploidy
Chromosomes, Human, Pair 7
*Chromosomes, Human, Pair 8
Data Interpretation, Statistical
Humans
In Situ Hybridization, Fluorescence
Interphase/genetics
Leukemia, Myeloid/genetics
*Monosomy/diagnosis
Normal Distribution
Poisson Distribution
Trisomy/*diagnosis
Pubmed
Web of science
Create date
25/01/2008 15:18
Last modification date
20/08/2019 15:18