Accounting for adjuvant-induced artifacts in the characterization of vaccine formulations by polyacrylamide gel electrophoresis.

Détails

ID Serval
serval:BIB_6148B6E2E31D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Accounting for adjuvant-induced artifacts in the characterization of vaccine formulations by polyacrylamide gel electrophoresis.
Périodique
Therapeutic Advances in Vaccines
Auteur(s)
Jakob V., Brunner L., Barnier-Quer C., Blust M., Collin N., Carter L., Carter D., Rausch K.M., Fox C.B.
ISSN
2051-0136 (Print)
ISSN-L
2051-0136
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
5
Numéro
2
Pages
31-38
Langue
anglais
Résumé
Several vaccine adjuvants comprise complex nano- or micro-particle formulations, such as oil-in-water emulsions. In order to characterize interactions and compatibility of oil-in-water emulsion adjuvants with protein antigens in vaccines, effective protein characterization methods that can accommodate potential interference from high concentrations of lipid-based particles are needed.
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein characterization technique which is affected by the presence of adjuvants such as oil-in-water emulsions. In this article, we investigate variations in SDS-PAGE methods that result in a reduction of adjuvant-induced staining artifacts. We have investigated whether the SDS method or the adjuvant composition were the reason for these artifacts and succeeded in reducing the artifacts with a modified sample preparation and different staining procedures.
The best results were obtained by using gold staining or silver staining instead of a Coomassie Blue staining procedure. Moreover, the replacement of the dilution buffer (20% SDS to disrupt emulsion) by alternative detergents such as Tween® 80 and Triton® X-100 removed adjuvant-induced streaking artifacts at the top of the gel.
These methods may be useful for improving characterization approaches of antigen-adjuvant mixtures by SDS-PAGE.

Pubmed
Open Access
Oui
Création de la notice
30/05/2017 18:00
Dernière modification de la notice
20/08/2019 15:18
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