Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control.

Details

Serval ID
serval:BIB_6117A2C8CEF8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control.
Journal
The Journal of cell biology
Author(s)
di Penta A., Mercaldo V., Florenzano F., Munck S., Ciotti M.T., Zalfa F., Mercanti D., Molinari M., Bagni C., Achsel T.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
09/02/2009
Volume
184
Number
3
Pages
423-435
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Messenger RNA (mRNA) transport to neuronal dendrites is crucial for synaptic plasticity, but little is known of assembly or translational regulation of dendritic messenger ribonucleoproteins (mRNPs). Here we characterize a novel mRNP complex that is found in neuronal dendrites throughout the central nervous system and in some axonal processes of the spinal cord. The complex is characterized by the LSm1 protein, which so far has been implicated in mRNA degradation in nonneuronal cells. In brain, it associates with intact mRNAs. Interestingly, the LSm1-mRNPs contain the cap-binding protein CBP80 that associates with (pre)mRNAs in the nucleus, suggesting that the dendritic LSm1 complex has been assembled in the nucleus. In support of this notion, neuronal LSm1 is partially nuclear and inhibition of mRNA synthesis increases its nuclear localization. Importantly, CBP80 is also present in the dendrites and both LSm1 and CBP80 shift significantly into the spines upon stimulation of glutamergic receptors, suggesting that these mRNPs are translationally activated and contribute to the regulated local protein synthesis.

Keywords
Animals, Biological Transport/physiology, Biomarkers/metabolism, Brain/cytology, Cells, Cultured, Cerebellum/cytology, Dendrites/physiology, Dendrites/ultrastructure, Endoribonucleases/genetics, Endoribonucleases/metabolism, Gene Expression Regulation, Gene Silencing, Mice, Mice, Inbred Strains, Neurons/cytology, Neurons/physiology, Nuclear Cap-Binding Protein Complex/genetics, Nuclear Cap-Binding Protein Complex/metabolism, Protein Biosynthesis, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins/metabolism, RNA, Messenger/metabolism, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Rats, Rats, Wistar, Ribonucleoproteins/genetics, Ribonucleoproteins/metabolism, Spinal Cord/cytology, mRNA Cleavage and Polyadenylation Factors/metabolism
Pubmed
Open Access
Yes
Create date
06/03/2017 17:23
Last modification date
20/08/2019 14:18
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