Actionable diagnosis of neuroleptospirosis by next-generation sequencing

Details

Serval ID
serval:BIB_60D422028531
Type
Article: article from journal or magazin.
Collection
Publications
Title
Actionable diagnosis of neuroleptospirosis by next-generation sequencing
Journal
N Engl J Med
Author(s)
Wilson M. R., Naccache S. N., Samayoa E., Biagtan M., Bashir H., Yu G., Salamat S. M., Somasekar S., Federman S., Miller S., Sokolic R., Garabedian E., Candotti F., Buckley R. H., Reed K. D., Meyer T. L., Seroogy C. M., Galloway R., Henderson S. L., Gern J. E., DeRisi J. L., Chiu C. Y.
ISSN
1533-4406 (Electronic)
ISSN-L
0028-4793
Publication state
Published
Issued date
2014
Volume
370
Number
25
Pages
2408-17
Language
english
Notes
Wilson, Michael R
Naccache, Samia N
Samayoa, Erik
Biagtan, Mark
Bashir, Hiba
Yu, Guixia
Salamat, Shahriar M
Somasekar, Sneha
Federman, Scot
Miller, Steve
Sokolic, Robert
Garabedian, Elizabeth
Candotti, Fabio
Buckley, Rebecca H
Reed, Kurt D
Meyer, Teresa L
Seroogy, Christine M
Galloway, Renee
Henderson, Sheryl L
Gern, James E
DeRisi, Joseph L
Chiu, Charles Y
eng
R01 HL105704/HL/NHLBI NIH HHS/
R01-HL105704/HL/NHLBI NIH HHS/
Howard Hughes Medical Institute/
Intramural NIH HHS/
Case Reports
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
N Engl J Med. 2014 Jun 19;370(25):2408-17. doi: 10.1056/NEJMoa1401268. Epub 2014 Jun 4.
Abstract
A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were negative. Targeted antimicrobial agents were administered, and the patient was discharged home 32 days later with a status close to his premorbid condition. Polymerase-chain-reaction (PCR) and serologic testing at the Centers for Disease Control and Prevention (CDC) subsequently confirmed evidence of Leptospira santarosai infection.
Keywords
Adenosine Deaminase/deficiency, Adolescent, Agammaglobulinemia/complications, Biopsy, Brain/*pathology, Cerebrospinal Fluid/*microbiology, DNA, Bacterial/*analysis, Fever/etiology, Headache/etiology, Humans, Leptospira/*genetics/isolation & purification, Leptospirosis/complications/*diagnosis/microbiology, Male, Meningoencephalitis/complications/*diagnosis/microbiology, Sequence Analysis, DNA/*methods, Severe Combined Immunodeficiency/complications
Pubmed
Create date
01/11/2017 10:29
Last modification date
20/08/2019 14:18
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